| Basic Science Investigative Resources |
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Cell and Tissue Culture facilities (3 laminar flow hoods, 6 incubators) Full Protein, DNA and RNA analysis Western blotting, ELISA and HPLC RT-PCR including quantitative, Northern and Southern Blotting, RNAi of target genes Lentiviral vector design and production
Imaging
Nikon TE-2000E Confocal Laser Microscopy Laser Intravital Microscopy Real-time cell analyses under flow Resin-embedding for Stented Vessel Sectioning
(With collaborators)
Olympus Two-photon microscopy Olympus Spectral Scanning Confocal Microscopy
Flow Cytometry
BD FACSCalibur 4-colour flow cytometer BD FACS Aria II 7-colour cell sorting
Small Animal Surgery Generation of bone marrow chimerics and transgenics Myocardial infarction; total occlusion or with reperfusion Angioplasty-mediated vascular injury
Image Gallery In vitro cell tunneling using high power laser confocal microscopy and 3D volume rendering with IMARIS software
Immunofluorescent staining of stented arteries
Intravital confocal microscopy
In vitro flow chamber applications
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