Basic Science Investigative Resources

Cell and Tissue Culture facilities (3 laminar flow hoods, 6 incubators)

Full Protein, DNA and RNA analysis

Western blotting, ELISA and HPLC

RT-PCR including quantitative,

Northern and Southern Blotting,

RNAi of target genes

Lentiviral vector design and production

 

Imaging

 

Nikon TE-2000E Confocal Laser Microscopy

Laser Intravital Microscopy

Real-time cell analyses under flow

Resin-embedding for Stented Vessel Sectioning

 

(With collaborators)

 

Olympus Two-photon microscopy

Olympus Spectral Scanning Confocal Microscopy

 

Flow Cytometry

 

BD FACSCalibur 4-colour flow cytometer

BD FACS Aria II 7-colour cell sorting

 

 

Small Animal Surgery

Generation of bone marrow chimerics and transgenics

Myocardial infarction; total occlusion or with reperfusion

Angioplasty-mediated vascular injury

 

 

 

 

Image Gallery

In vitro cell tunneling using high power laser confocal microscopy and 3D volume rendering with IMARIS software

 

In-Vitro-Tunneling

 

Immunofluorescent staining of stented arteries

Pig-coro-stent

Intravital confocal microscopy

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In vitro flow chamber applications

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